Application of a ImageJ-Based Method to Measure Blood Flow in Adult Zebrafish and Its Applications for Toxicological and Pharmacological Assessments

Blood flow is an important physiological endpoint to measure cardiovascular performance in animals.Because of their innate transparent bodies, zebrafish is an excellent animal model for assessing in vivo cardiovascular performance.Previously, various helpful methods for measuring blood flow in zebrafish larvae were discovered and developed.However,

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Panax ginseng-Derived Extracellular Vesicles Facilitate Anti-Senescence Effects in Human Skin Cells: An Eco-Friendly and Sustainable Way to Use Ginseng Substances

Ginseng is a traditional herbal medicine in eastern Asian countries.Most active constituents in ginseng are prepared via fermentation or organic acid pretreatment.Extracellular vesicles (EVs) are released by most organisms from prokaryotes to eukaryotes and play central roles in intra- and inter-species communications.Plants produce EVs upon exposu

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An ER Protein Functionally Couples Neutral Lipid Metabolism on Lipid Droplets to Membrane Lipid Synthesis in the ER

Eukaryotic cells store neutral lipids such as triacylglycerol (TAG) in lipid droplets (LDs).Here, we have addressed how LDs are functionally linked to the endoplasmic reticulum (ER).We show Display Cabinet that, in S.cerevisiae, LD growth is sustained by LD-localized enzymes.When LDs grow in early stationary phase, the diacylglycerol acyl-transfera

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Budget impact analysis of routinely using whole-genomic sequencing of six multidrug-resistant bacterial pathogens in Queensland, Australia

Objective To predict the cost and health effects of routine use of whole-genome sequencing (WGS) of bacterial pathogens compared with those of standard of care.Design Budget impact analysis was performed over the following 5 years.Data were primarily from sequencing results on clusters of multidrug-resistant organisms across 27 hospitals.Model inpu

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